{"id":20,"date":"2012-01-11T00:54:58","date_gmt":"2012-01-11T00:54:58","guid":{"rendered":"https:\/\/arabidopsisunpak.org\/?page_id=20"},"modified":"2025-04-15T19:41:13","modified_gmt":"2025-04-15T19:41:13","slug":"the-biology","status":"publish","type":"page","link":"https:\/\/arabidopsisunpak.org\/?page_id=20","title":{"rendered":"The Biology"},"content":{"rendered":"<p><strong>About <em>Arabidopsis thaliana<\/em><\/strong><\/p>\n<p style=\"padding-left: 30px;\"><em>Arabidopsis thaliana<\/em>\u00a0is a small, fast-growing, flowering plant that is used as a model system in many areas of biology. \u00a0What makes Arabidopsis such an amazing biological tool? \u00a0Click on the link to find out more: <a title=\"About Arabidopsis thaliana\" href=\"https:\/\/arabidopsisunpak.org\/?page_id=316\">[About Arabidopsis thaliana]<\/a><\/p>\n<p><strong>About T-DNA insertion mutant\u00a0plant lines<\/strong><\/p>\n<p style=\"padding-left: 30px;\">Plant genetics utilizes a collection of \u2018knockout\u2019 mutant strains in the model species\u00a0<em>Arabidopsis thaliana<\/em>. Knockouts, carrying a tiny tagged segment of disruptive DNA (T-DNA) in one targeted gene, are already available for ~67% of the 27,000+ unique genes in this plant\u2019s genome. \u00a0Our goal is to collect fitness data for as many of these mutant strains as possible. \u00a0Read more about the T-DNA insertion lines: <a title=\"Arabidopsis T-DNA Insertion Lines\" href=\"https:\/\/arabidopsisunpak.org\/?page_id=77\">[About T-DNA insertional lines]<\/a> .<\/p>\n<p><strong>Phenotyping mutant lines<\/strong><\/p>\n<p style=\"padding-left: 30px;\"><span style=\"font-weight: 400;\">One of the goals of the unPAK project is to record phenotypes of Arabidopsis T-DNA single knockout mutants grown in many different environmental conditions. Multiple genes can be involved in a single phenotype and environmental conditions may alter how genes behave. Arabidopsis mutants can be experimentally grown under different environmental conditions to screen for environmental and genotypic interactions that may affect phenotypic traits.<\/span><\/p>\n<p><strong>Genotyping insertion lines<\/strong><\/p>\n<p style=\"padding-left: 30px;\">The SALK T-DNA mutant collection is a important and powerful tool used in plant genetic research. \u00a0The goal of the unPAK project is to investigate the phenotypic effect of knocking out single genes from the Arabidopsis genome. \u00a0As part of the unPAK project we are using a simple PCR-based procedure to ensure the lines we use have only a single disrupted gene.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>About Arabidopsis thaliana Arabidopsis thaliana\u00a0is a small, fast-growing, flowering plant that is used as a model system in many areas of biology. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":10,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-20","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/arabidopsisunpak.org\/index.php?rest_route=\/wp\/v2\/pages\/20","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/arabidopsisunpak.org\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/arabidopsisunpak.org\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/arabidopsisunpak.org\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/arabidopsisunpak.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=20"}],"version-history":[{"count":10,"href":"https:\/\/arabidopsisunpak.org\/index.php?rest_route=\/wp\/v2\/pages\/20\/revisions"}],"predecessor-version":[{"id":1476,"href":"https:\/\/arabidopsisunpak.org\/index.php?rest_route=\/wp\/v2\/pages\/20\/revisions\/1476"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/arabidopsisunpak.org\/index.php?rest_route=\/wp\/v2\/media\/10"}],"wp:attachment":[{"href":"https:\/\/arabidopsisunpak.org\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=20"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}