Investigating Arabidopsis Plant Lines: Mutants

SALK T-DNA MUTANTS

To find more information about your natural population start at the Arabidopsis website: www.arabidopsis.org

Step 1: When you arrive at the TAIR (The Arabidopsis Information Resource) page search for your line.  At the top right of the screen enter in your mutant # (SALK_XXXXX), select Germplasm from the search menu and hit “SEARCH.”

 

Step 2: Your search should return a page similar to this (here we searched for SALK_122845C).  Click on the linked text of the SALK # for more information about the line and/or the linked text of the locus (in this case it is AT2G47420) to find more about the gene your mutation should be in.

 

Also, visit the T-DNA Express page for more information about your mutant plant line.

Step 1: Go to T-DNA Express: www. signal.salk.edu.  The front page will look similar to the image below – type in your mutant # in the Query box (NOTE: DO NOT ADD THE “C” AT THE END – THIS WILL RESULT IN A FAILED SEARCH).  Hit “Search.”

 

Step 2:  Your search result will bring you to the place where your mutation is located.  At the top of the page in green you will see the different Arabidopsis genes as they are physically located in the Arabidopsis genome.  The genes are represented by green bars (the arrowhead indicates the direction) – solid bars are exons, the interruptions represent introns.  Below that map is a collection of smaller arrows of various colors (all described by matching color text).  Look for your mutant # – it should be represented by a green arrow.  Once you find it, click on the mutant # – it will bring you to a new page with more information.

 

Step 3: Clicking on your mutant number will bring you to a new text page with more information about the line.  Here you can find the exact coordinates of the insert (for this mutant, chromosome 2 19,457,472-19,457,788 base pairs) and the location within the gene (for this mutant, 300-UTR5′ which means it is in the 5′ untranslated region (UTR) of the gene).

Other links of interest:

  1. Information about background line (Col) as well as Landsberg and Ws: NASC Collection Information

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